Journal: AACE Clinical Case Reports

Article Title: Hypercalcemia of Malignancy Attributed to Cosecretion of PTH and PTHRP in Lung Adenocarcinoma

doi: 10.1016/j.aace.2021.01.003

Figure Lengend Snippet: Tissue from a brain metastasis resected a month prior to admission, showing positive nuclear staining for PTHrP (dark brown). Paraffin sections were dewaxed, rehydrated, and antigen retrieval was performed in 10 mM sodium citrate, pH 6.0, for 10 minutes using an electric pressure cooker. Sections were cooled, blocked with 3% hydrogen peroxide, then for endogenous biotin (Biocare Medical Kit #AB972), and incubated with 10% goat serum. Rabbit polyclonal antibody to PTHrP (sc-20728; Santa Cruz Biotechnology, Santa Cruz, CA) was used at 1:200 with overnight incubation at 4 o C. PTHrP antibody binding was detected using the Vectastain Elite ABC HRP Kit (Vector Labs, Burlingame, CA) and developed with Dako Liquid DAB substrate (Dako Corp, Carpinteria, CA). PTHrP = parathyroid hormone-related peptide.

Article Snippet: Rabbit polyclonal antibody to PTHrP (sc-20728; Santa Cruz Biotechnology, Santa Cruz, CA) was used at 1:200 with overnight incubation at 4 o C. PTHrP antibody binding was detected using the Vectastain Elite ABC HRP Kit (Vector Labs, Burlingame, CA) and developed with Dako Liquid DAB substrate (Dako Corp, Carpinteria, CA).

Techniques: Staining, Incubation, Binding Assay, Plasmid Preparation

Journal: AACE Clinical Case Reports

Article Title: Hypercalcemia of Malignancy Attributed to Cosecretion of PTH and PTHRP in Lung Adenocarcinoma

doi: 10.1016/j.aace.2021.01.003

Figure Lengend Snippet: Overview of the Mechanisms and Frequency of Hypercalcemia of Malignancy

Article Snippet: Rabbit polyclonal antibody to PTHrP (sc-20728; Santa Cruz Biotechnology, Santa Cruz, CA) was used at 1:200 with overnight incubation at 4 o C. PTHrP antibody binding was detected using the Vectastain Elite ABC HRP Kit (Vector Labs, Burlingame, CA) and developed with Dako Liquid DAB substrate (Dako Corp, Carpinteria, CA).

Techniques:

Journal: BioMed Research International

Article Title: Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy

doi: 10.1155/2018/6947612

Figure Lengend Snippet: Immunostaining changes of condylar cartilage. The PTHrP positive cells were distributed in the proliferative and early hypertrophic layers (a–c). Ihh was expressed in the extracellular space and concentrated within the proliferative layer (d–f). The Col2a1 positive area was shown in the hyperplastic layers; the intensity and area predicted the matrix's volume (g–i) ( ∗p<0.05;∗∗p<0.01;∗∗∗p<0.001 ). Bar=25 μ m.

Article Snippet: The primary antibodies were PTHrP (1:200, SC-20728, Santa Cruz Biotechnology, Inc.), Ihh (1:250, AB-52919, Abcam), and Col 2 α 1 (1:100, SC-7763, Santa Cruz Biotechnology, Inc.).

Techniques: Immunostaining

Journal: BioMed Research International

Article Title: Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy

doi: 10.1155/2018/6947612

Figure Lengend Snippet: Protein fold change in cartilage after treatment. Western blot analysis of condylar cartilage. PTHrP and Ihh showed a similar declination after decreased occlusal loading treatment (b, c). The two extracellular collagens, Col2a1 and Col X, were more sensitive to the altered loading, which resulted in a dramatic decrease in the BTX group (d, e) ( ∗p<0.05;∗∗p<0.01;∗∗∗p<0.001 ).

Article Snippet: The primary antibodies were PTHrP (1:200, SC-20728, Santa Cruz Biotechnology, Inc.), Ihh (1:250, AB-52919, Abcam), and Col 2 α 1 (1:100, SC-7763, Santa Cruz Biotechnology, Inc.).

Techniques: Western Blot